CD40 stimulation activates CD8+ T cells and controls HBV in CD4-depleted mice.

ABSTRACT:

Background & aims

HBV treatment is challenging due to the persistence of the covalently closed circular DNA replication pool, which remains unaffected by antiviral intervention. In this study, we determined whether targeting antigen-presenting cells via CD40 stimulation represents an appropriate therapeutic approach for achieving sustained HBV control in a mouse model of HBV replication.

Methods

Mice were transduced with an adeno-associated virus encoding the HBV genome (AAV-HBV) to initiate HBV replication and were administered agonistic CD40 antibody. CD4-depleting antibody was administered in addition to the CD40 antibody. Viral antigens in the blood were measured over time to determine HBV control. HBV-specific CD8⁺ T cells were quantified in the spleen and li

SUBMITTER: Bailey JT 

PROVIDER: S-EPMC11399595 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature