Optimal liquid-based DNA preservation for DNA barcoding of field-collected fungal specimens.

ABSTRACT: Preserving fungal tissue DNA in the field is essential for molecular ecological research, enabling the study of fungal biodiversity and community dynamics. This study systematically compares two liquid-based preservation solutions, RNAlater and DESS, for their effectiveness in maintaining macrofungi DNA integrity during field collection and storage. The research encompasses both controlled experiments and real-world field collections. In the controlled experiments, two fungal species were preserved in RNAlater and DESS at different temperatures and durations. DNA extraction success rates were high, but DNA quality and quantity metrics exhibited variations across samples. However, both preservation solutions demonstrated their viability for preserving fungal DNA, with no significant differe

SUBMITTER: Lee YJ 

PROVIDER: S-EPMC11401026 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature