EEF1B2 regulates the proliferation and apoptosis of human spermatogonial stem cell lines through TAF4B.

ABSTRACT:

Background

Spermatogonial stem cells (SSCs) are essential for male fertility, maintaining sperm production throughout life. While mouse SSCs have been studied extensively, the mechanisms regulating human SSCs are less understood.

Objectives

To investigate the role of EEF1B2 in regulating human SSC proliferation and apoptosis.

Material and methods

Single cell RNA sequencing (scRNA-seq) analysis was utilized to investigate the differentially expressed genes of SSC. The distribution of EEF1B2 in the human testis was examined using immunofluorescence and immunohistochemistry techniques. Cell proliferation, DNA replication, and self-renewal were analyzed using CCK8, EdU, Western blot, and flow cytometry. RNA sequencing was employed to analyze the downstream target molecule

SUBMITTER: Huang Z 

PROVIDER: S-EPMC11401128 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature