A new generation of nanobody research tools using improved mass spectrometry-based discovery methods.

ABSTRACT: Single-domain antibodies ("nanobodies") derived from the variable region of camelid heavy-chain only antibody variants have proven to be widely useful tools for research, therapeutic, and diagnostic applications. In addition to traditional display techniques, methods to generate nanobodies using direct detection by mass spectrometry and DNA sequencing have been highly effective. However, certain technical challenges have limited widespread application. We have optimized a new pipeline for this approach that greatly improves screening sensitivity, depth of antibody coverage, antigen compatibility, and overall hit rate and affinity. We have applied this improved methodology to generate significantly higher affinity nanobody repertoires against widely used targets in biological research-i.e.,

SUBMITTER: Fridy PC 

PROVIDER: S-EPMC11401214 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature