Faecal microbiota and cytokine profiles of rural Cambodian infants linked to diet and diarrhoeal episodes.

ABSTRACT: The gut microbiota of infants in low- to middle-income countries is underrepresented in microbiome research. This study explored the faecal microbiota composition and faecal cytokine profiles in a cohort of infants in a rural province of Cambodia and investigated the impact of sample storage conditions and infant environment on microbiota composition. Faecal samples collected at three time points from 32 infants were analysed for microbiota composition using 16S rRNA amplicon sequencing and concentrations of faecal cytokines. Faecal bacterial isolates were subjected to whole genome sequencing and genomic analysis. We compared the effects of two sample collection methods due to the challenges of faecal sample collection in a rural location. Storage of faecal samples in a DNA preservation so

SUBMITTER: Dalby MJ 

PROVIDER: S-EPMC11401897 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature