Improved functionality of hepatic spheroids cultured in acoustic levitation compared to existing 2D and 3D models.

ABSTRACT: Hepatic spheroids are of high interest in basic research, drug discovery and cell therapy. Existing methods for spheroid culture present advantages and drawbacks. An alternative technology is explored: the hepatic spheroid formation and culture in an acoustofluidic chip, using HepaRG cell line. Spheroid formation and morphology, cell viability, genetic stability, and hepatic functions are analyzed after 6 days of culture in acoustic levitation. They are compared to 2D culture and non-levitated 3D cultures. Sizes of the 25 spheroids created in a single acoustofluidic microphysiological system are homogeneous. The acoustic parameters in our system do not induce cell mortality nor DNA damage. Spheroids are cohesive and dense. From a functional point of view, hepatic spheroids obtained by acou

SUBMITTER: Rabiet L 

PROVIDER: S-EPMC11401944 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature