Screening and identification of nanobody against inhibin α-subunit from a Camelus bactrianus phage display library.

ABSTRACT:

Background

Inhibin is a member of the transforming growth factor family that influences reproduction in animals.

Objective

The purpose of this study was to obtain nanobodies from the phage antibody library constructed by us that can specifically bind to inhibin α-subunit.

Methods

In this study, camels were immunized with Kazakh sheep inhibin-α protein that expressed in BL21 E. coli, and the camel VHH nanobody phage display library was prepared using nested PCR. The nanobodies specifically binding to inhibin α-subunit in the library were screened by three rounds of immunoaffinity screening and phage enzyme-linked immunosorbent assay (phage ELISA). The functions of the selected nanobodies were identified using molecular simulation docking, ELISA affinity test, an

SUBMITTER: Ma J 

PROVIDER: S-EPMC11402152 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature