Plug-and-play nucleic acid-mediated multimerization of biparatopic nanobodies for molecular imaging.

ABSTRACT: In cancer molecular imaging, selecting binders with high specificity and affinity for biomarkers is paramount for achieving high-contrast imaging within clinical time frames. Nanobodies have emerged as potent candidates, surpassing antibodies in pre-clinical imaging due to their convenient production, rapid renal clearance, and deeper tissue penetration. Multimerization of nanobodies is a popular strategy to enhance their affinity and pharmacokinetics; however, traditional methods are laborious and may yield heterogeneous products. In this study, we employ a Holliday junction (HJ)-like nucleic acid-based scaffold to create homogeneous nanostructures with precise multivalent and multiparatopic nanobody displays. The plug-and-play assembly allowed the screening of several nanobody multimer c

SUBMITTER: Teodori L 

PROVIDER: S-EPMC11402398 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature