Complex sphingolipid profiling and identification of an inositol-phosphorylceramide synthase in Dictyostelium discoideum.

ABSTRACT: Dictyostelium discoideum is a professional phagocyte frequently used to study cellular processes underlying the recognition, engulfment, and infection course of microbial pathogens. Sphingolipids are abundant components of the plasma membrane that bind cholesterol, control membrane properties, participate in signal transmission, and serve as adhesion molecules in recognition processes relevant to immunity and infection. By combining lipidomics with a bioinformatics-based cloning strategy, we show here that D. discoideum produces phosphoinositol-containing sphingolipids with predominantly phytoceramide backbones. Cell-free expression of candidate inositol-phosphorylceramide (IPC) synthases from D. discoideum enabled identification of an enzyme that selectively catalyzes

SUBMITTER: Listian SA 

PROVIDER: S-EPMC11402645 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature