Exploiting branched-chain amino acid metabolism and NOTCH3 expression to predict and target colorectal cancer progression.

ABSTRACT:

Background

The interplay between colon adenocarcinoma (COAD) and branched-chain amino acid (BCAA) metabolism is not fully understood, presenting a crucial area for investigation.

Methods

We developed a prognostic model based on BCAA metabolism using the least absolute shrinkage and selection operator (LASSO) regression algorithm. We employed qRT-PCR and Western blot analyses to examine NOTCH3 expression in COAD tissues versus adjacent non-cancerous tissues and various cell lines. We also investigated the impact of NOTCH3 on COAD cell proliferation, invasion, and migration through in vitro and in vivo experiments.

Results

Our BCAA metabolism-related signature (BRS) distinguished between different immune features, tumor mutation burdens, responses to immun

SUBMITTER: Shen K 

PROVIDER: S-EPMC11402679 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature