Cell-free protein synthesis with technical additives - expanding the parameter space of in vitro gene expression.

ABSTRACT: Biocatalysis has established itself as a successful tool in organic synthesis. A particularly fast technique for screening enzymes is the in vitro expression or cell-free protein synthesis (CFPS). The system is based on the transcription and translation machinery of an extract-donating organism to which substrates such as nucleotides and amino acids, as well as energy molecules, salts, buffer, etc., are added. After successful protein synthesis, further substrates can be added for an enzyme activity assay. Although mimicking of cell-like conditions is an approach for optimization, the physical and chemical properties of CFPS are not well described yet. To date, standard conditions have mainly been used for CFPS, with little systematic testing of whether conditions closer to intracellular c

SUBMITTER: Bartsch T 

PROVIDER: S-EPMC11403795 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature