The two-component signal transduction system RR06/HK06 regulates expression of cbpA in Streptococcus pneumoniae.

ABSTRACT: Streptococcus pneumoniae encounters a number of environmental niches in the body, including the nasopharynx, lungs, blood, middle ear, and brain. Recent studies have identified 13 putative two-component signal-transduction systems in S. pneumoniae, which are likely to be important for gene regulation in response to external stimuli. Here, we present conclusive evidence for the regulation of choline binding protein A (CbpA), a major pneumococcal virulence factor and protective antigen, by one of these two-component signal-transduction systems. We have demonstrated divergent expression of cbpA in unmarked hk06 and rr06 deletion mutants relative to wild-type S. pneumoniae D39 by using Western immunoblotting and real-time RT-PCR. Electrophoretic mobility-shift and solid-phase binding assays ha

SUBMITTER: Standish AJ 

PROVIDER: S-EPMC1140415 | biostudies-literature | 2005 May

REPOSITORIES: biostudies-literature