A comparative study of the efficacy of alginate lyases in the presence of metal ions elevated in the cystic fibrosis lung milieu.

ABSTRACT: Pseudomonas aeruginosa, a common cause of morbidity in cystic fibrosis, chronically infects the patient's lungs by forming an alginate-containing biofilm. Alginate lyases are polysaccharide lyases that lyse alginate and are, therefore, potential biofilm-disruptive agents. However, cystic fibrosis sputum contains high levels of metals such as iron and zinc. The efficacy of alginate lyases under these conditions of elevated metal concentrations has not been categorically determined. Here, we have assessed the enzyme activity of two exolytic and five endolytic alginate lyases in the presence of metal ions (Fe²⁺, Zn²⁺, Mn²⁺, Mg²⁺, Ca²⁺, Ni²⁺, Cu²⁺) elevated in the cystic fibrosis lung milieu. Several of these a

SUBMITTER: Neetu 

PROVIDER: S-EPMC11404220 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature