Sub-zero non-freezing of vascularized composite allografts in a rodent partial hindlimb model.

ABSTRACT: Ischemia is a major limiting factor in Vascularized Composite Allotransplantation (VCA) as irreversible muscular injury can occur after as early as 4-6 h of static cold storage (SCS). Organ preservation technologies have led to the development of storage protocols extending rat liver ex vivo preservation up to 4 days. Development of such a protocol for VCAs has the added challenge of inherent ice nucleating factors of the graft, therefore, this study focused on developing a robust protocol for VCA supercooling. Rodent partial hindlimbs underwent subnormothermic machine perfusion (SNMP) with several loading solutions, followed by a storage solution with cryoprotective agents (CPA) developed for VCAs. Storage occurred in suspended animation for 24h and VCAs were recovered using SNMP with mod

SUBMITTER: Filz von Reiterdank I 

PROVIDER: S-EPMC11404353 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature