Quantitating intracellular transport of polyplexes by spatio-temporal image correlation spectroscopy.

ABSTRACT: Quantitatively understanding how nonviral gene delivery vectors (polyplexes) are transported inside cells is essential before they can be optimized for gene therapy and medical applications. In this study, we used spatio-temporal image correlation spectroscopy (ICS) to follow polymer-nucleic acid particles (polyplexes) of various sizes and analyze their diffusive-like and flow behaviors intracellularly to elucidate the mechanisms responsible for their transport. ICS is a quantitative imaging technique that allows the assessment of particle motion in complex systems, although it has not been widely used to date. We find that the internalized polyplexes are able to use microtubule motors for intracellular trafficking and exhibit different transport behaviors for short (<10 s) versus long (ap

SUBMITTER: Kulkarni RP 

PROVIDER: S-EPMC1140437 | biostudies-literature | 2005 May

REPOSITORIES: biostudies-literature