Repeated glucose oscillations in high cell-density cultures influence stress-related functions of Escherichia coli.

ABSTRACT: Engineering microbial cells for the commercial production of biomolecules and biochemicals requires understanding how cells respond to dynamically changing substrate (feast-famine) conditions in industrial-scale bioreactors. Scale-down methods that oscillate substrate are commonly applied to predict the industrial-scale behavior of microbes. We followed a compartment modeling approach to design a scale-down method based on the simulation of an industrial-scale bioreactor. This study uses high cell-density scale-down experiments to investigate Escherichia coli knockout strains of five major glucose-sensitive transcription factors (Cra, Crp, FliA, PrpR, and RpoS) to study their regulatory role during glucose oscillations. RNA-sequencing analysis revealed that the glucose oscillations

SUBMITTER: Bafna-Ruhrer J 

PROVIDER: S-EPMC11404509 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature