ECT2 peptide sequences outside the YTH domain regulate its m⁶A-RNA binding.

ABSTRACT: The m⁶A epitranscriptomic mark is the most abundant and widespread internal RNA chemical modification, which through the control of RNA acts as an important factor of eukaryote reproduction, growth, morphogenesis and stress response. The main m⁶A readers constitute a super family of proteins with hundreds of members that share a so-called YTH RNA binding domain. The majority of YTH proteins carry no obvious additional domain except for an Intrinsically Disordered Region (IDR). In Arabidopsis thaliana IDRs are important for the functional specialization among the different YTH proteins, known as Evolutionarily Conserved C-Terminal region, ECT 1 to 12. Here by studying the ECT2 protein and using an in vitro biochemical characterization, we show that

SUBMITTER: Seigneurin-Berny D 

PROVIDER: S-EPMC11404569 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature