Segmental patterning of microbiota and immune cells in the murine intestinal tract.

ABSTRACT: The intestine exhibits distinct characteristics along its length, with a substantial immune cell reservoir and diverse microbiota crucial for maintaining health. This study investigates how anatomical location and regional microbiota influence intestinal immune cell abundance. Using conventionally colonized and germ-free mice, segment-specific immune cell composition and microbial communities were assessed. Metagenomic sequencing analyzed microbiome variations, while flow cytometry and immunofluorescence examined immune cell composition. Microbiome composition varied significantly along the intestine, with diversity and abundance increasing from upper to lower segments. Immune cells showed distinct segment-specific patterning influenced by microbial colonization and localization. T cell su

SUBMITTER: Anandakumar H 

PROVIDER: S-EPMC11404582 | biostudies-literature | 2024 Jan-Dec

REPOSITORIES: biostudies-literature