Phosphorylation-Regulated Dynamic Phase Separation of HIP-55 Protects Against Heart Failure.

ABSTRACT:

Background

Heart failure (HF), which is the terminal stage of many cardiovascular diseases, is associated with low survival rates and a severe financial burden. The mechanisms, especially the molecular mechanism combined with new theories, underlying the pathogenesis of HF remain elusive. We demonstrate that phosphorylation-regulated dynamic liquid-liquid phase separation of HIP-55 (hematopoietic progenitor kinase 1-interacting protein of 55 kDa) protects against HF.

Methods

Fluorescence recovery after photobleaching assay, differential interference contrast analysis, pull-down assay, immunofluorescence, and immunohistochemical analysis were used to investigate the liquid-liquid phase separation capacity of HIP-55 and its dynamic regulation in vivo and in vitro. Mice with ge

SUBMITTER: Jiang Y 

PROVIDER: S-EPMC11404759 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature