Effects of platelet-rich fibrin on human endometrial stromal cells behavior in comparison to platelet-rich plasma.

ABSTRACT:

Introduction

Intrauterine transfusion of platelet-rich plasma (PRP) has become a new treatment for thin endometrium (TE) in recent years, but its low efficacy due to rapid release of growth factors limits its clinical use. Platelet-rich fibrin (PRF) starts the coagulation cascade reaction immediately after the blood comes into contact with the test tube. The natural coagulation process results in stable platelet activation and the slow release of growth factors.

Methods

In our study, primary human endometrial stromal cells (hESCs) were extracted from endometrial tissue. PRP and PRF were prepared from the patient cubital vein blood. Stromal cells were cultured in conditioned medium supplemented with PRP and PRF. Differences in cell behavior were observed by cell proliferation

SUBMITTER: Yuan G 

PROVIDER: S-EPMC11405248 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature