Using grass inflorescence as source material for biomonitoring through environmental DNA metabarcoding.

ABSTRACT:

Background

Over the last decade, increasing attention has been directed to using different substrates as sources of environmental DNA (eDNA) in ecological research. Reports on the use of environmental DNA located on the surface of plant leaves and flowers have highlighted the utility of this DNA source in studies including, but not limited to, biodiversity, invasive species, and pollination ecology. The current study assesses grass inflorescence as a source of eDNA for detecting invertebrate taxa.

Methods and results

Inflorescences from four common grass species in a central South African grassland were collected for high-throughput sequencing analysis. Universal COI primers were utilised to detect Metazoan diversity. The sequencing results allowed for the detection of three

SUBMITTER: Coetzer WG 

PROVIDER: S-EPMC11405429 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature