Mechanism of sensory perception unveiled by simultaneous measurement of membrane voltage and intracellular calcium.

ABSTRACT: Measuring neuronal activity is important for understanding neuronal function. Ca²⁺ imaging by genetically encoded calcium indicators (GECIs) is a powerful way to measure neuronal activity. Although it revealed important aspects of neuronal function, measuring the neuronal membrane voltage is important to understand neuronal function as it triggers neuronal activation. Recent progress of genetically encoded voltage indicators (GEVIs) enabled us fast and precise measurements of neuronal membrane voltage. To clarify the relation of the membrane voltage and intracellular Ca²⁺, we analyzed neuronal activities of olfactory neuron AWA in Caenorhabditis elegans by GCaMP6f (GECI) and paQuasAr3 (GEVI) responding to odorants. We found that the membrane voltage encodes the stimul

SUBMITTER: Tokunaga T 

PROVIDER: S-EPMC11405522 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature