A simple method for repeated in vivo sperm collection from laboratory mice.

ABSTRACT:

Purpose

Mouse spermatozoa for archiving laboratory mice or for in vitro fertilization (IVF) are routinely obtained from the cauda epididymis of adult males sacrificed for this purpose. To avoid the death of the donor, we tested whether a precisely timed interruption of the mating act could be used for repeated sperm collection from laboratory mice.

Methods

Sperm donors (B6D2F1) were mated with a receptive female, and mating behavior was observed. The stud was separated from the female 1-2 s after the onset of the ejaculatory shudder. The ejected copulatory plug with the yellowish viscous ejaculate was carefully removed from the penile cup.

Results

A total of 80 ejaculates were successfully obtained from 100 ejaculations. The latency to first mount was 1.1 ± 1.1 min (m

SUBMITTER: Burgstaller SM 

PROVIDER: S-EPMC11405545 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature