Enrichment of cell cycle pathways in progesterone-treated endometrial organoids of infertile women compared to fertile women.

ABSTRACT:

Purpose

To investigate whether the transcriptome profile differs between progesterone-treated infertile and fertile endometrial organoids.

Methods

Endometrial biopsies were obtained from 14 infertile and seven fertile women, after which organoids were generated from isolated epithelial cells. To mimic the secretory phase, organoids were sequentially treated with 17β-estradiol (E2) and progesterone (P4) and subjected to RNA sequencing. Differentially expressed genes (DEGs) were identified using DESeq2 (lfcThreshold = 0, log₂ Fold Change ≥ 1.0 or ≤ -1.0), and a principal component analysis (PCA) plot was generated. Functional enrichment analysis was performed by overrepresentation analysis and Gene Set Enrichment Analysis (GSEA). To functionally assess proliferation

SUBMITTER: Bui BN 

PROVIDER: S-EPMC11405558 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature