Deciphering the role of rapamycin in modulating decidual senescence: implications for decidual remodeling and implantation failure.

ABSTRACT:

Purpose

Physiological decidual senescence promotes embryo implantation, whereas pathological decidual senescence causes many pregnancy pathologies. The aim of this study was to evaluate the effect of rapamycin on decidual cell subpopulations and endometrial function in physiological and induced senescence and to investigate the decidual cell subpopulations present in physiological conditions during early pregnancy and implantation in mice.

Methods

Control, physiological decidualization (0.5 mM cAMP and 1 μM MPA added), and induced senescence (0.1 mM HU added) models with and without 200 nM rapamycin treatment were established using a human endometrial stromal cell line, and decidual cell subpopulations were analyzed by immunofluorescence and flow cytometry. The human extravi

SUBMITTER: Kendirci-Katirci R 

PROVIDER: S-EPMC11405573 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature