3D bioprinted breast cancer model reveals stroma-mediated modulation of extracellular matrix and radiosensitivity.

ABSTRACT: Deciphering breast cancer treatment resistance remains hindered by the lack of models that can successfully capture the four-dimensional dynamics of the tumor microenvironment. Here, we show that microextrusion bioprinting can reproducibly generate distinct cancer and stromal compartments integrating cells relevant to human pathology. Our findings unveil the functional maturation of this millimeter-sized model, showcasing the development of a hypoxic cancer core and an increased surface proliferation. Maturation was also driven by the presence of cancer-associated fibroblasts (CAF) that induced elevated microvascular-like structures complexity. Such modulation was concomitant to extracellular matrix remodeling, with high levels of collagen and matricellular proteins deposition by CAF, simu

SUBMITTER: Desigaux T 

PROVIDER: S-EPMC11405629 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature