Highly efficient CRISPR-mediated genome editing through microfluidic droplet cell mechanoporation.

ABSTRACT: Clustered regularly interspaced short palindromic repeats (CRISPR)-based editing tools have transformed the landscape of genome editing. However, the absence of a robust and safe CRISPR delivery method continues to limit its potential for therapeutic applications. Despite the emergence of various methodologies aimed at addressing this challenge, issues regarding efficiency and editing operations persist. We introduce a microfluidic gene delivery system, called droplet cell pincher (DCP), designed for highly efficient and safe genome editing. This approach combines droplet microfluidics with cell mechanoporation, enabling encapsulation and controlled passage of cells and CRISPR systems through a microscale constriction. Discontinuities created in cell and nuclear membranes upon passage faci

SUBMITTER: Kim YJ 

PROVIDER: S-EPMC11405868 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature