De novo engineering of programmable and multi-functional biomolecular condensates for controlled biosynthesis.

ABSTRACT: There is a growing interest in the creation of engineered condensates formed via liquid-liquid phase separation (LLPS) to exert precise cellular control in prokaryotes. However, de novo design of cellular condensates to control metabolic flux or protein translation remains a challenge. Here, we present a synthetic condensate platform, generated through the incorporation of artificial, disordered proteins to realize specific functions in Bacillus subtilis. To achieve this, the "stacking blocks" strategy is developed to rationally design a series of LLPS-promoting proteins for programming condensates. Through the targeted recruitment of biomolecules, our investigation demonstrates that cellular condensates effectively sequester biosynthetic pathways. We successfully harness this capability t

SUBMITTER: Yu W 

PROVIDER: S-EPMC11405872 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature