Efficient and cost-effective differentiation of induced neural crest cells from induced pluripotent stem cells using laminin 211.

ABSTRACT:

Introduction

Neural crest cells (NCCs) are cell populations that originate during the formation of neural crest in developmental stages. They are characterized by their multipotency, self-renewal and migration potential. Given their ability to differentiate into various types of cells such as neurons and Schwann cells, NCCs hold promise for cell therapy applications. The conventional method for obtaining NCCs involves inducing them from stem cells like induced pluripotent stem cells (iPSCs), followed by a long-term passage or purification using fluorescence-activated cell sorting (FACS). Although FACS allows high purity induced neural crest cells (iNCCs) to be obtained quickly, it is complex and costly. Therefore, there is a need for a simpler, cost-effective and less time-consumin

SUBMITTER: Takahashi K 

PROVIDER: S-EPMC11406167 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature