4D intravital imaging studies identify platelets as the predominant cellular procoagulant surface in a mouse hemostasis model.

ABSTRACT:

Abstract

Interplay between platelets, coagulation factors, endothelial cells (ECs), and fibrinolytic factors is necessary for effective hemostatic plug formation. This study describes a 4-dimensional (4D) imaging platform to visualize and quantify hemostatic plug components in mice with high spatiotemporal resolution. Fibrin accumulation after laser-induced vascular injury was observed at the platelet plug-EC interface, controlled by the antagonistic balance between fibrin generation and breakdown. We observed less fibrin accumulation in mice expressing low levels of tissue factor or F12-/-mice compared with controls, whereas increased fibrin accumulation, including on the vasculature adjacent to the platelet plug, was observed in plasminogen-deficient mice or wild-type mice treate

SUBMITTER: Ballard-Kordeliski A 

PROVIDER: S-EPMC11406176 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature