Integrative single-cell chromatin and transcriptome analysis of human plasma cell differentiation.

ABSTRACT:

Abstract

Plasma cells (PCs) are highly specialized cells representing the end stage of B-cell differentiation. We have shown that PC differentiation can be reproduced in vitro using elaborate culture systems. The molecular changes occurring during PC differentiation are recapitulated in this in vitro differentiation model. However, a major challenge exists to decipher the spatiotemporal epigenetic and transcriptional programs that drive the early stages of PC differentiation. We combined single cell (sc) RNA sequencing (RNA-seq) and assay for transposase-accessible chromatin with high throughput sequencing (scATAC-seq) to decipher the trajectories involved in PC differentiation. ScRNA-seq experiments revealed a strong heterogeneity of the preplasmablastic and plasmablastic stages.

SUBMITTER: Alaterre E 

PROVIDER: S-EPMC11406183 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature