High-level succinic acid production by overexpressing a magnesium transporter in <i>Mannheimia succiniciproducens</i>.

ABSTRACT: Succinic acid (SA), a dicarboxylic acid of industrial importance, can be efficiently produced by metabolically engineered Mannheimia succiniciproducens. Although the importance of magnesium (Mg²⁺) ion on SA production has been evident from our previous studies, the role of Mg²⁺ ion remains largely unexplored. In this study, we investigated the impact of Mg²⁺ ion on SA production and developed a hyper-SA producing strain of M. succiniciproducens by reconstructing the Mg²⁺ ion transport system. To achieve this, optimal alkaline neutralizer comprising Mg²⁺ ion was developed and the physiological effect of Mg²⁺ ion was analyzed. Subsequently, the Mg²⁺ ion transport system was reconstructed by introducing

SUBMITTER: Kim JY 

PROVIDER: S-EPMC11406231 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature