Promoters Constrain Evolution of Expression Levels of Essential Genes in Escherichia coli.

ABSTRACT: Variability in expression levels in response to random genomic mutations varies among genes, influencing both the facilitation and constraint of phenotypic evolution in organisms. Despite its importance, both the underlying mechanisms and evolutionary origins of this variability remain largely unknown due to the mixed contributions of cis- and trans-acting elements. To address this issue, we focused on the mutational variability of cis-acting elements, that is, promoter regions, in Escherichia coli. Random mutations were introduced into the natural and synthetic promoters to generate mutant promoter libraries. By comparing the variance in promoter activity of these mutant libraries, we found no significant difference in mutational variability in promoter activity between promoter groups, s

SUBMITTER: Tsuru S 

PROVIDER: S-EPMC11406756 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature