Optimization of ribosome profiling in plants including structural analysis of rRNA fragments.

ABSTRACT:

Background

Ribosome profiling (or Ribo-seq) is a technique that provides genome-wide information on the translational landscape (translatome). Across different plant studies, variable methodological setups have been described which raises questions about the general comparability of data that were generated from diverging methodologies. Furthermore, a common problem when performing Ribo-seq are abundant rRNA fragments that are wastefully incorporated into the libraries and dramatically reduce sequencing depth. To remove these rRNA contaminants, it is common to perform preliminary trials to identify these fragments because they are thought to vary depending on nuclease treatment, tissue source, and plant species.

Results

Here, we compile valuable insights gathered over years

SUBMITTER: Ting MKY 

PROVIDER: S-EPMC11406806 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature