Structured multicellular intestinal spheroids (SMIS) as a standardized model for infection biology.

ABSTRACT:

Background

3D cell culture models have recently garnered increasing attention for replicating organ microarchitecture and eliciting in vivo-like responses, holding significant promise across various biological disciplines. Broadly, 3D cell culture encompasses organoids as well as single- and multicellular spheroids. While the latter have found successful applications in tumor research, there is a notable scarcity of standardized intestinal models for infection biology that mimic the microarchitecture of the intestine. Hence, this study aimed to develop structured multicellular intestinal spheroids (SMIS) specifically tailored for studying molecular basis of infection by intestinal pathogens.

Results

We have successfully engineered human SMIS comprising four relevant cell typ

SUBMITTER: Kraski A 

PROVIDER: S-EPMC11406839 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature