The storage time of cryopreserved human spermatozoa does not affect pathways involved in fertility.

ABSTRACT:

Background

Cryopreservation of human spermatozoa is a widely used technique in the assisted reproduction technology laboratory for the storage of gametes for later use, for the fertility preservation and for sperm donation programs. Cryopreservation can cause damage to membrane, cytoskeletal, acrosome and increased oxidative stress, sperm DNA damage and transcriptome changes. To assess the impact of storage time on the transcriptome of frozen human spermatozoa, semen samples were collected from 24 normospermic donors of whom 13 had cryostored semen for a short-time (1 week) and 11 had cryostored semen for a long-time (median 9 years).

Results

RNA was extracted from each frozen-thawed sperm sample, randomized in pools, and analyzed by microarrays. Five transcripts were in hig

SUBMITTER: Stigliani S 

PROVIDER: S-EPMC11406918 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature