Interspecies synergistic interactions mediated by cofactor exchange enhance stress tolerance by inducing biofilm formation.

ABSTRACT: Metabolic exchange plays a crucial role in shaping microbial community interactions and functions, including the exchange of small molecules such as cofactors. Cofactors are fundamental to enzyme catalytic activities; however, the role of cofactors in microbial stress tolerance is unclear. Here, we constructed a synergistic consortium containing two strains that could efficiently mineralize di-(2-ethylhexyl) phthalate under hyperosmotic stress. Integration of transcriptomic analysis, metabolic profiling, and a genome-scale metabolic model (GEM) facilitated the discovery of the potential mechanism of microbial interactions. Multi-omics analysis revealed that the vitamin B₁₂-dependent methionine-folate cycle could be a key pathway for enhancing the hyperosmotic stress tolerance of

SUBMITTER: Wang L 

PROVIDER: S-EPMC11406921 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature