Afucosylated anti-EBOV antibody MIL77-3 engages sGP to elicit NK cytotoxicity.

ABSTRACT: MIL77-3 is one component of antibody cocktail that is produced in our lab and represents an effective regimen for animals suffering from Zaire Ebolavirus (EBOV) infection. MIL77-3 is engineered to increase its affinity for the FcγRIIIa (CD16a) by deleting the fucose in the framework region. The potential effects of this modification on host immune responses, however, remain largely unknown. Herein, we demonstrated that MIL77-3 recognized secreted glycoproptein (sGP), produced by EBOV, and formed the immunocomplex to potently augment antibody-dependent cytotoxicity of human peripheral blood-derived natural killer cells (pNKs), including CD56^dim and CD56^bright subpopulations, in contrast to the counterparts (Mab114, rEBOV548, fucosylated MIL77-3). Intriguingly, this eff

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC11406966 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature