A reporter Oropouche virus expressing ZsGreen from the M segment enables pathogenesis studies in mice.

ABSTRACT: Oropouche fever caused by Oropouche virus (OROV) is a significant zoonosis in Central and South America. Despite its public health significance, we lack high-throughput diagnostics, therapeutics, and a comprehensive knowledge of OROV biology. Reporter viruses are valuable tools to rapidly study virus dynamics and develop neutralization and antiviral screening assays. OROV is a tri-segmented bunyavirus, which makes generating a reporter virus challenging, as introducing foreign elements into the viral genome typically affects fitness. We previously demonstrated that the non-structural gene NSm on the OROV medium (M) segment is non-essential for replication in vitro. Taking advantage of this, we have now generated a recombinant OROV expressing fluorescent protein ZsGreen in place of N

SUBMITTER: Gunter K 

PROVIDER: S-EPMC11406970 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature