In vivo fitness of sul gene-dependent sulfonamide-resistant Escherichia coli in the mammalian gut.

ABSTRACT: The widespread sulfonamide resistance genes sul1, sul2, and sul3 in food and gut bacteria have attracted considerable attention. In this study, we assessed the in vivo fitness of sul gene-dependent sulfonamide-resistant Escherichia coli, using a murine model. High fitness costs were incurred for sul1 and sul3 gene-dependent E. coli strains in vivo. A fitness advantage was found in three of the eight mice after intragastric administration of sul2 gene-dependent E. coli strains. We isolated three compensatory mutant strains (CMSs) independently from three mice that outcompeted the parent strain P2 in vivo. Whole-genome sequencing revealed seven identical single nucleotide polymorphism (SNP) mutations i

SUBMITTER: Jiang H 

PROVIDER: S-EPMC11406977 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature