The Rab6 post-Golgi secretory pathway contributes to herpes simplex virus 1 (HSV-1) egress.

ABSTRACT: Herpes simplex virus 1 (HSV-1) is an alpha herpesvirus that infects a majority of the world population. The mechanisms and cellular host factors involved in the intracellular transport and exocytosis of HSV-1 particles are not fully understood. To elucidate these late steps in the replication cycle, we developed a live-cell fluorescence microscopy assay of HSV-1 virion intracellular trafficking and exocytosis. This method allows us to track individual virus particles and identify the precise moment and location of particle exocytosis using a pH-sensitive reporter. We show that HSV-1 uses the host cell's post-Golgi secretory pathway during egress. The small GTPase, Rab6, binds to nascent secretory vesicles at the trans-Golgi network and plays important, but non-essential, roles in ve

SUBMITTER: Bergeman MH 

PROVIDER: S-EPMC11406995 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature