In silico full-angle high-dynamic range scattering of microscopic objects exploiting holotomography.

ABSTRACT: Accurate optical characterization of microscopic objects is crucial in academic research, product development, and clinical diagnosis. We present a method for obtaining full and high-dynamic range, angle-resolved light scattering attributes of microparticles, enabling distinction of variations in both overall morphology and detailed internal structures. This method overcomes previous limitations in observable scattering angles and dynamic range of signals through computationally assisted three-dimensional holotomography. This advancement is significant for particles spanning tens of wavelengths, such as human erythrocytes, which have historically posed measurement challenges due to faint side-scattering signals indicative of their complex interiors. Our technique addresses three key challe

SUBMITTER: Kang SK 

PROVIDER: S-EPMC11407242 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature