Harnessing Interactional Sensory Genes for Rationally Reprogramming Chaotic Metabolism.

ABSTRACT: Rationally controlling cellular metabolism is of great importance but challenging owing to its highly complex and chaotic nature. Natural existing sensory proteins like histidine kinases (HKs) are understood as "sensitive nodes" of biological networks that can trigger disruptive metabolic reprogramming (MRP) upon perceiving environmental fluctuation. Here, the "sensitive node" genes were adopted to devise a global MRP platform consisting of a CRISPR interference-mediated dual-gene combinational knockdown toolbox and survivorship-based metabolic interaction decoding algorithm. The platform allows users to decode the interfering effects of n × n gene pairs while only requiring the synthesis of n pairs of primers. A total of 35 HK genes and 24 glycine metabolic genes were

SUBMITTER: Tan C 

PROVIDER: S-EPMC11407584 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature