Multifocal microscopy for functional imaging of neural systems.

ABSTRACT:

Significance

Rapid acquisition of large imaging volumes with microscopic resolution is an essential unmet need in biological research, especially for monitoring rapid dynamical processes such as fast activity in distributed neural systems.

Aim

We present a multifocal strategy for fast, volumetric, diffraction-limited resolution imaging over relatively large and scalable fields of view (FOV) using single-camera exposures.

Approach

Our multifocal microscopy approach leverages diffraction to image multiple focal depths simultaneously. It is based on a custom-designed diffractive optical element suited to low magnification and large FOV applications and customized prisms for chromatic correction, allowing for wide bandwidth fluorescence imaging. We integrate this system w

SUBMITTER: Meitav N 

PROVIDER: S-EPMC11407684 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature