High throughput and rapid isolation of extracellular vesicles and exosomes with purity using size exclusion liquid chromatography.

ABSTRACT: Extracellular vesicles (EVs) have emerged as potential biomarkers for diagnosing a range of diseases without invasive procedures. Extracellular vesicles also offer advantages compared to synthetic vesicles for delivery of various drugs; however, limitations in segregating EVs from other particles and soluble proteins have led to inconsistent EV retrieval rates with low levels of purity. Here, we report a new high-yield (88.47 %) and rapid (<20 min) EV isolation method termed size exclusion - fast protein liquid chromatography (SE-FPLC). We show SE-FPLC can effectively isolate EVs from multiple sources including EVs derived from human and mouse cells and serum samples. The results indicate that SE-FPLC can successfully remove highly abundant protein contaminants such as albumin and lipoprot

SUBMITTER: Kapoor KS 

PROVIDER: S-EPMC11407901 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature