Peptidomic profiling of endogenous peptides in the spleens of mouse models of Graves' disease.

ABSTRACT:

Background

Graves' disease (GD) is a common autoimmune thyroid disorder. The pathogenesis of GD involves an autoimmune response to the A subunit of the human thyrotropin receptor (hTSHR), although the specific mechanisms are not fully elucidated.

Methods

This study established a GD model by immunizing BALB/c mice with a recombinant adenovirus expressing the hTSHR A subunit. Spleen tissues were collected and analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) to identify differentially expressed peptides (DEPs). Gene Ontology (GO) analysis and KEGG pathway analysis were further utilized to annotate the functions of these DEPs. Additionally, peptide bioactivity prediction and molecular docking studies were conducted using Alphafold and Pymol software, resp

SUBMITTER: Jiang Z 

PROVIDER: S-EPMC11408014 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature