Performance comparison of high throughput single-cell RNA-Seq platforms in complex tissues.

ABSTRACT: Single-cell transcriptomics has emerged as the preferred tool to define cell identity through the analysis of gene expression signatures. However, there are limited studies that have comprehensively compared the performance of different scRNAseq systems in complex tissues. Here, we present a systematic comparison of two well-established high throughput 3'-scRNAseq platforms: 10× Chromium and BD Rhapsody, using tumours that present high cell diversity. Our experimental design includes both fresh and artificially damaged samples from the same tumours, which also provides a comparable dataset to examine their performance under challenging conditions. The performance metrics used in this study consist of gene sensitivity, mitochondrial content, reproducibility, clustering capabilities, cell ty

SUBMITTER: Colino-Sanguino Y 

PROVIDER: S-EPMC11408078 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature