CyuR is a dual regulator for L-cysteine dependent antimicrobial resistance in Escherichia coli.

ABSTRACT: Hydrogen sulfide (H₂S), mainly produced from L-cysteine (Cys), renders bacteria highly resistant to oxidative stress and potentially increases antimicrobial resistance (AMR). CyuR is a Cys-dependent transcription regulator, responsible for the activation of the cyuPA operon and generation of H₂S. Despite its potential importance, its regulatory network remains poorly understood. In this study, we investigate the roles of the CyuR regulon in a Cys-dependent AMR mechanism in E. coli strains. We show: (1) Generation of H₂S from Cys affects the sensitivities to growth inhibitors; (2) Cys supplementation decreases stress responses; (3) CyuR negatively controls the expression of mdlAB encoding a potential transporter for antibiotics; (4) CyuR binds to a DNA seque

SUBMITTER: Rodionova IA 

PROVIDER: S-EPMC11408624 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature