How storage post sampling influences the stability of sebum when used for mass spectrometry metabolomics analysis?

ABSTRACT: Sebum is a biofluid excreted by sebaceous glands in the skin. In recent years sebum has been shown to contain endogenous metabolites diagnostic of disease, with remarkable results for Parkinson's Disease. Given that sebum sampling is facile and non-invasive, its potential for use in clinical biochemistry diagnostic assays should be explored including the parameters for standard operating procedures around collection, transport, and storage. To this aim we have here investigated the reproducibility of mass spectrometry data from sebum in relation to both storage temperature and length of storage. Sebum samples were collected from volunteers and stored for up to four weeks at a range of temperatures: ambient (circa 17 °C), -20 °C and -80 °C. Established extraction protocols were employed and

SUBMITTER: Walton-Doyle C 

PROVIDER: S-EPMC11408688 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature