Prognostic significance of immune-cell distribution and tumoral spread through air spaces - Multiplex spatial immunophenotyping analysis.

ABSTRACT:

Objectives

Spread through air spaces (STAS) is a form of lung cancer invasion that extends beyond the tumor edge and is associated with a worse prognosis. Recent advances in immunotherapy highlight the importance of understanding the tumor microenvironment. This study aimed to investigate the prognostic significance of immune-cell distribution in lung cancer, focusing on the association with STAS.

Materials and methods

We retrospectively analyzed 283 patients who underwent curative-intent lung resection for primary lung cancer. Multiplex immunofluorescence staining/phenotyping was performed on tissue microarrays to assess the distribution of CD4, CD8, CD20, CD68, and FoxP3 immune cells within the center and tumor edge. We defined the delta-Edge value (Δ) as the difference in

SUBMITTER: Matsuoka S 

PROVIDER: S-EPMC11408789 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature